Giemsa stain can also be used to stain films of unformed faeces, faecal exudate, duodenal aspirates etc. Method 1. Make a thin film of faeces/exudate. Allow to dry. 2. Fix in methanol for 1 min. 3. Tip off the methanol and flood the slide with Giemsa stain (Code – 1484) diluted 1:10 with buffered distilled [...]
Method 1. Make faecal smears as for ZN and fix in methanol. 2. Stain with Auramine-Phenol (Lemperts) for 10-15 min. 3. Rinse thoroughly in tap water. 4. Decolourise in acid alcohol (as for ZN). 5. Rinse thoroughly in tap water. 6. Counterstain with 0.1% potassium permanganate for 30 sec. 7. Rinse thoroughly in tap water, [...]
The addition of acridine orange to a faecal concentrate highlights the chromidial bars of Entamoeba coli, Entamoeba histolytica/ dispar and Entamoeba hartmanni, which fluoresce bright green. Working solution Stock acridine orange - 1 ml Glacial acetic acid – 0.5 ml Buffered water pH 6.8 - 8.5 ml Method Add an equal volume of stain to [...]
This stain is useful for the detection of motile trophozoites of Entamoeba species. Method Emulsify faeces directly in a warm 37°C solution of eosin in saline. The amoebae are easily seen unstained against a pink background. The coarse, granular endoplasm can be differentiated from the clear, colourless ectoplasm.
Mayer’s glycerin – albumin is used when preparing slides for staining. The albumin helps to ensure that the specimen adheres to the slide and the glycerin retains sufficient moisture to prevent distortion or disruption of organisms on drying. Method 1. Shake the Mayer’s Albumin to ensure it is well mixed. Place a drop in the [...]
