Biochemical Oxygen Demand (BOD)
The BOD test is an indirect measure of the biodegradable organic matter in an aqueous sample. The test is indirect because oxygen used by microbes as they degrade organic matter is measured. Generally the test is performed over a 5 day period (BOD5) of 20°C in 300 ml bottles incubated in the dark to prevent interference from algal growth. Dissolved oxygen (DO) levels (in mg/L) are measured in day 0 and at the end of the test.
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